As a new clean energy source, the demands for and use of hydrogen fuel are rapidly increasing. Therefore, biohydrogen production technology is being developed to reduce operation costs in many countries. Improvement of biohydrogen production capacity and cost reduction are key factors to bring about industrial implementation. One of the most effective production methods is microbiological: the use of bacteria with high hydrogen-production capacity and performance. The anaerobic process of biohydrogen production was developed in the 1990s. The isolation and identification of highly efficient biohydrogen producing anaerobic bacteria is an important foundation for the fermentative production of hydrogen by anaerobic digestion of organic wastewater.

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